Sequential multi-stage extraction of biocompounds from Spirulina platensis: Combined effect of ohmic heating and enzymatic treatment

A sequential multi-stage procedure was applied on the extraction of biocompounds from Spirulina platensis. The process consisted in three steps: 1) aqueous extraction, using conventional thermal (CE), ohmic heating (OH, 7 V/cm), enzymatic treatment (EAE, 0.8 mgLysozyme/mL), or both OH and EAE combined; 2) ethanolic extraction; 3) CHCl3/MeOH extraction. results evidenced that combined OH-EAE allowed selective recovery phycobiliproteins 1st step, with increments more than 100% yield comparison CE. Pigments lipids were selectively extracted 2nd step. combination step resulted higher amounts compounds following phases compared to processes non-combined technologies. Results demonstrate intensification steps facilitates use environmentally friendly technologies a capable recovering isolating different fractions bio-functional properties, targeting waste reduction circular economy. plantensis represents potential biomass feedstock due its as source great economic value (including antioxidants, proteins, natural pigments, particular blue colorants). enzymes fosters implement high purity valuable contributing implementation economy strategies. This can be integrated design Industry 4.0 driving development new products.